Introduction
to Instruct-ERIC
Instruct-ERIC is an European distributed Research Infrastructure that provides open access to high-end structural biology services and techniques (including sample characterization, X-ray crystallography, NMR and cryo-EM, among others), making cutting-edge technologies and methods available to European researchers. Technologies and services are free for the user, since they are entirely funded by the Instruct platform.
Sample Preparation
Cristallization
Crystallisation allows the 3D structure of macromolecules to be revealed through X-ray diffraction. Instruct offer a fully automated crystallisation pipeline to achieve high-throughput with short crystallisation plate processing times, high reproducibility, and increased efficiency of the screening process.
Nanobody Discovery
Nanobodies are single chain antibodies which have revolutionary applications in structural biology. Our Nanobody Discovery service is accessible to all Instruct researchers.
Protein Induction
Instruct’s services include protein expression, cloning and high throughput expression, and protein purification. Our techniques allow for expression of challenging proteins along with expert protein purification systems.
Biomolecular Analysis
Imaging
Imaging techniques including fluorescence microscopy provide an efficient and unique approach to study fixed and living cells because of their versatility, specificity, and high sensitivity.
Mass Spectometry
Mass spectrometry is the dominant technology in the field of proteomics, enabling the identification and quantification of cellular proteins and their modified forms.
Molecular Biophysics
Instruct offers a wide range of techniques to study macromolecular interactions, including circular dichroism, surface plasmon resonance (SPR), thermal shift assay and calorimetry.
3d Structural Analysis
Electron Microscopy
The high-resolution electron microscope has evolved into a sophisticated instrument that is capable of routinely providing quantitative structural information on the atomic scale.
Magnetic Resonance Techniques
NMR allows three-dimensional structural and dynamic information to be obtained in conditions as close as possible to physiological ones. Functional processes can be followed in living cells, and transient protein-protein interactions can be investigated.
X-Ray Techniques
Instruct-ERIC offers a wide range of X ray approaches to determine the three dimensional shape of proteins at the atomic level.
How to apply
Access all the information related to Instruct and their services